NExTLi
The National Expert Center for Translational Lipidomics
A coordinated Dutch research infrastructure enabling structurally resolved, spatially anchored, and flux-informed lipidomics — with expert guidance and FAIR-by-design data handling to make mechanistic lipid biology feasible at national scale.
What NExTLi enables
- Structural lipidomics: classes, species, isomers
- Low-input workflows (scarce material, small regions)
- Spatial integration (imaging/MSI-aware design)
- Metabolic tracing / flux-informed interpretation
- FAIR deliverables and portal-ready packaging
Why NExTLi
Many projects can generate lipid profiles, but mechanistic lipid biology often fails on structure, context, and dynamics. NExTLi brings those capabilities together as a shared infrastructure — with expert guidance and standardized outputs.
How it works
- Intake: question, samples, constraints, desired outputs
- Design: structural / spatial / flux strategy
- Standardized processing and QC
- Expert-supported analysis and interpretation
- FAIR packaging for reuse and integration
Three capability pillars
Structural
Workflows designed for higher-confidence lipid annotation — including isomer-informed strategies when the biology requires it.
Spatial
Study design that links lipid measurements to regions, cell types, and imaging/MSI-aware sampling strategies.
Flux
Tracer-informed experiments and interpretation that connect lipid changes to pathway dynamics and causality.