NExTLi
The National Expert Center for Translational Lipidomics
A coordinated Dutch research infrastructure enabling in-depth lipidomics — integrating structural resolution, spatial anchoring, and metabolic flux. NExTLi is designed for studies where sample amount is limiting (scarce material, small regions) and interpretation requires standardized, expert-guided workflows and FAIR-by-design outputs.
What NExTLi enables
- Structural lipidomics: classes, species, isomers
- Low-input workflows (scarce material, small regions)
- Spatial integration (imaging/MSI-aware design)
- Metabolic tracing / flux-informed interpretation
- FAIR deliverables and portal-ready packaging
Why NExTLi
Many projects can generate lipid profiles, but mechanistic lipid biology often fails on structure, context, and dynamics — especially when samples are small or spatially defined. NExTLi brings these capabilities together as a shared infrastructure with standardized QC and expert-supported interpretation.
How it works
- Intake: question, samples, constraints, desired outputs
- Design: structural / spatial / flux strategy (incl. low-input feasibility)
- Standardized processing, QC and documentation
- Expert-supported analysis and interpretation
- FAIR packaging for reuse and integration
Three capability pillars
Structural
Workflows designed for higher-confidence lipid annotation — including isomer-informed strategies when the biology requires it.
Spatial
Study design that links lipid measurements to regions, cell types, and imaging/MSI-aware sampling strategies — including small-region feasibility.
Flux
Tracer-informed experiments and interpretation that connect lipid changes to pathway dynamics and causality.