NExTLi
The National Expert Center for Translational Lipidomics
A coordinated Dutch research infrastructure enabling in-depth lipidomics — integrating structural lipid resolution, spatial anchoring, and metabolic flux. NExTLi is designed for studies where sample amount is limiting (scarce material, small regions) and interpretation requires structural confidence, spatial context, and flux-informed interpretation, delivered through standardized, expert-guided workflows and FAIR-by-design outputs.
What NExTLi enables
- In-depth lipidomics: classes and species
- Low-input workflows (scarce material, small regions)
- Spatial integration (imaging/MSI-aware design)
- Metabolic tracing / flux-informed interpretation
- FAIR deliverables and portal-ready packaging
Why NExTLi
Many projects can generate lipid profiles, but mechanistic lipid biology often fails on structure, context, and dynamics — especially when samples are small or spatially defined. NExTLi brings these capabilities together as a shared infrastructure with standardized QC and expert-supported interpretation.
How it works
- Intake: question, samples, constraints, desired outputs
- Design: in-depth / spatial / flux strategy (incl. low-input feasibility)
- Standardized processing, QC and documentation
- Expert-supported analysis and interpretation
- FAIR packaging for reuse and integration
Three capability pillars
Structural
Workflows designed for high-confidence lipid annotation with structural resolution when required.
Spatial
Study design that links lipid measurements to regions, cell types, and imaging/MSI-aware sampling strategies — including small-region feasibility.
Flux
Tracer-informed experiments and interpretation that connect lipid changes to pathway dynamics and causality.